Cloned (Comment) | Organism |
---|---|
removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-amino-1,3-cyclohexadienylcarboxylate | - |
Malus domestica | |
Aminooxyacetate | - |
Malus domestica | |
beta-Alanine | moderate inhibitor; moderate inhibitor | Malus domestica | |
ornithine | moderate inhibitor; moderate inhibitor | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0165 | - |
glyoxylate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
0.0188 | - |
pyruvate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
0.023 | - |
pyruvate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
0.039 | - |
glyoxylate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
0.077 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: pyruvate | Malus domestica | |
0.099 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: glyoxylate | Malus domestica | |
0.135 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: glyoxylate | Malus domestica | |
0.165 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: pyruvate | Malus domestica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Malus domestica | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54200 | - |
SDS-PAGE, single recombinant truncated MdGABA-T | Malus domestica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | J9XGP8 | - |
- |
Malus domestica | J9XGZ5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + glyoxylate | - |
Malus domestica | 4-oxobutanoate + glycine | - |
? | |
4-aminobutanoate + pyruvate | - |
Malus domestica | 4-oxobutanoate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GABA-T | - |
Malus domestica |
gamma-aminobutyrate transaminase | - |
Malus domestica |
MdGABA-T1 | - |
Malus domestica |
MdGABA-T2 | - |
Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
glyoxylate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
0.8 | - |
pyruvate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
0.84 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: pyruvate | Malus domestica | |
1 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: glyoxylate | Malus domestica | |
2.6 | - |
glyoxylate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
2.6 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: pyruvate | Malus domestica | |
2.7 | - |
pyruvate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
2.7 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: glyoxylate | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Malus domestica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | maximal activity | Malus domestica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Malus domestica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.4 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: glyoxylate | Malus domestica | |
10.9 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: pyruvate | Malus domestica | |
16.1 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: pyruvate | Malus domestica | |
19.5 | - |
4-aminobutanoate | pH 9, 25°C, fixed substrate: glyoxylate | Malus domestica | |
42.7 | - |
pyruvate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
45.7 | - |
glyoxylate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
66.6 | - |
glyoxylate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica | |
116 | - |
pyruvate | pH 9, 25°C, fixed substrate: 4-aminobutanoate | Malus domestica |